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Cynomolgus GDF-15 / MIC-1 Protein, His Tag

分子别名(Synonym)

GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1

表达区间及表达系统(Source)

Cynomolgus GDF-15, His Tag (GD5-C5148) is expressed from E.coli cells. It contains AA Ala 197 - Val 308 (Accession # G7PWZ3-1).

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 197 - Val 308) G7PWZ3-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.4 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Cynomolgus GDF-15, His Tag (Cat. No. GD5-C5148) SDS-PAGE gel

Cynomolgus GDF-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus GDF-15, His Tag (Cat. No. GD5-C5148) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like Fc Chimera Protein with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

背景(Background)

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

 

前沿进展

 
 
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GDF-15靶点信息
英文全称:Growth/differentiation factor 15
中文全称:生长分化因子-15
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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